Methods
To address the scientific questions we are concerned with we mainly employ cellular immunological techniques as well as cell culture and animal models of human diseases:
- lymphocyte separation using magnetic or flow cytometric sorting
- cell culture of primary lymphocytes isolated from humans, mice or pigs
- multi-color flow cytometry
- cytotoxicity tests
- histology and immunohistochemistry
- generation of monoclonal antibodies with the hybridoma method
- disease models: acute myocardial infarction, systemic infections with Candida albicans, Measles virus infection of the brain