Methods applied
Methods applied:
Cellular Immunology:
Immuno-flow-cytometry (FACS), cytokine-, proliferation- and cytotoxicity tests, generation of T- and B-cell hybridomas (monoclonal antibodies), purification and labeling of antibodies.
Molecular Biology and Biochemistry:
Cloning and expression of immunologically relevant genes, gene-transduction and knockout (CRISPR/Cas9), immunoprecipitation and Western-blot, generation of CD1d-oligomers, confocal microscopy, FRAP, FRET